blast

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1-TOPO vector (Invitrogen, Frederick, MD, USA), and selected clones from the same cloning reaction were sequenced 3 times with a ABI Prism 377 automated sequencer (Perkin Elmer, Foster City, CA, USA), and the sequences were compared to those in the GenBank database by using the Basic Local Alignment Search Tool at the National Center for Biotechnology Information (12).
Ann Arbor MI, USA), and basic local alignment search tool (BLAST) searches were managed on the NCBI Web site (http://www.

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