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culture |
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Although the specimen exhibited enterovirus-like cytopathic effect by inoculation into HEF and GMK cells when cell culture system for virus isolation was used (2), extracted RNA from the supernatant of the cells showed no amplification by reverse transcription--PCR (RT-PCR) when 1 of the conventional primer sets for human enteroviruses was used (3,4). From these cells the scientists then teased out dopamine cells in a stable culture system that was later transferred into mice. When a cow is in the hospital pen, Baker cultures her milk using an on-farm milk test, the Minnesota Easy Culture System. |
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