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Sequences for 500 subcloned cDNAs from a single degenerate 3'RACE reaction were analyzed and provided insight into the utility of a degenerate primer in terms of the maximum number of unique sequences that can be amplified simultaneously.
It is concluded that the degenerate primers can be used for isolation of new gene fragments from fungi.
In this regard, development of degenerate primers would be a valuable tool in identifying the other populations of B.
To isolate the 5' eDNA fragment of the isolated NII561-2000, the eDNA fragment containing part of the 5' untranslated region (UTR) and capsid precursor protein VP0 was amplified by PCR from the cDNA prepared from the NII561-2000 virus with degenerate primers corresponding to this region.
RT-PCR amplification of total RNA from the soybean pod borer 3rd instar gut tissues using degenerate primers (from the regions of conserved amino acids flanking the active site of the His75 and Ser 220 residues) amplified a 400 bp fragment.
The degenerate primers CLCV1/CLCV2 and specific primers (PadCPPVXF/PadCPPVXR) were used for detection of ToLCNDV (Table I).
Although MY09/11 degenerate primers are routinely and largely used to detect mucosal HPV (Alpha species), especially from genital mucosa, the FAP 59/64 system has been applied in the detection of cutaneous HPV (Beta and Gamma species) and has been used to improve detection of oral HPV (12) The combination of these systems tracks a broad range of types, even types not yet identified.
2011) reported that an immune capture reverse transcription (IC-RT)PCR assay employing degenerate primers for amplifying partial coat protein (CP).
Identification of begomovirus components by PCR PCR was performed using a pair of degenerate primers specific to the coat protein region of begomovirus.
DNA from a novel herpesvirus was amplified from lung tissue by polymerase chain reaction by using degenerate primers derived from conserved avian herpesvirus sequences.