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Since degenerate primers used in this amplification, more than one fragment is expected to be amplified.
Signal amplification Target amplification Example HC2 (29) PCR MY09/11 (30) Probes/primers Mixture of RNA probes Degenerate primers Test reaction product DNA/RNA hybrids 450 bp Analytical 25 to 75 0.
striatellus by means of degenerate primers, specific primers were designed and used to amplify the full length sequence.
To confirm adenovirus infection, we used a PCR assay with degenerate primers designed from conserved regions of the adenovirus DNA-polymerase gene.
We performed PCRs with degenerate primers (CV-F1, CV-R1, CV-F2, and CV-R2; Table 1) based on highly conserved amino acid motifs in the Rep proteins of circoviruses and cycloviruses.
Degenerate primers, Bd1, 5'-ATGAARTTYG GNWSNTGGACNTAYGA-3' and Bd2, 5'-GCNACCATRAACATDATRCARTTRAA-3', Were designed based on the conserved motifs of nicotinic acetylcholine receptor family to amplify the cDNA fragments (Gao et al.
The 5' and 3' untranslated regions were obtained by nested PCR with combined specific primers and henipavirus-specific degenerate primers as described (8), and the exact sequences of the 5' and 3' genome termini were determined by rapid amplification of cDNA ends.
All rabbit serum and fecal swab samples were tested for HEV RNA by using a nested reverse transcription PCR and a set of degenerate primers that amplify a conserved region of the HEV capsid gene.
Detection of HEV RNA was performed by nested reverse transcription-PCR with 2 sets of degenerate primers that targeted open reading frame 1 (ORF1) and the overlapping part of ORF2 and ORF3 (ORF2/3) of the HEV genome as described previously (6).
Nested reverse transcription-PCR using degenerate primers was applied to amplify a 222-bp fragment of the large RNA segment of phleboviruses (9).