Purification and crystallization of the yeast elongation factor eEF2.
Endogenous ADP-ribosylation for eukaryotic elongation factor 2: evidence of two different sites and reactions.
The researchers' analysis also suggests that the way poxA and yjeK modify the bacterial protein elongation factor
is essential in the production of proteins that allow the bacteria to use alternative energy sources when they are deprived of nutrients, as occurs after they enter host cells.
Some of the identified targets are: Caspase 3, Colony stimulating factor 2, COX, Cyclin-dependent kinase inhibitor 1A, DNA (cytosine-5-)-methyltransferase 1, Epidermal growth factor receptor, Eukaryotic translation elongation factor
1 alpha 1 etc.
The SS1P molecule is an ingenious example of protein engineering: the native Pseudomonas exotoxin protein consists of three domains: domain I targets cell surface receptors, domain II functions to translocate the molecule across a cell membrane and into the cell interior, and domain III catalyzes irreversible ADP ribosylation and inactivation of elongation factor