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Embryonic Stem Cells Generated Without Use Of Embryos Or Retroviral Vectors To Add Exogenous DNA To Host Cells
Toward that end we developed the episomal reprogramming method to ensure that the resulting iPSCs are free of exogenous DNA, and this CIRM grant validates that method as researchers worldwide will access the resulting iPSC lines for disease research.
Today, the most widely used methods for the production of transgenic farm animals are direct microinjection of foreign DNA into the pronuclei of fertilized eggs, nuclear transfer using genetically modified embryonic or somatic donor cells and viral-based constructs as vectors for the introduction of exogenous DNA into embryos.
As such, the invention may be used for the insertion of exogenous DNA sequences into the bacteria of the invention.
Aerosol-resistant tips were used throughout the protocol to prevent contamination with exogenous DNA.
However, concerns about exogenous DNA permanently incorporated into the cells have limited development in this area.
Cambridge, MA; 617-349-0271) announced the United States Patent and Trademark Office has issued United States Patent 6,187,305 relating to a gene therapy method where exogenous DNA is inserted into vertebrate cells in vitro through a process called homologous recombination, also known as gene targeting.