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After treatment with HCl, the slides were rinsed in running tap water for 5 s and dried before staining with the aceto-orcein and Giemsa stains, as described above.
The Duet(TM) had previously received FDA clearance for Hematopoietic cells stained by Giemsa stain, Immunohistochemistry or ISH (with bright field and fluorescent) prepared from cell suspension and Amniotic cells stained by FISH (using direct labeled DNA probes for chromosomes X, Y, 13, 18 and 21).
1,2) The identification of leishmania is possible by Giemsa stain touch imprint cytology and/or specific antibodies on tissue.
The central scale was removed from one of the lesions, and scrapings from the base of the lesion were stained with Giemsa stain, which showed intracellular amastigotes with peripheral nuclei and rod-shaped kinetoplasts.
Slides were brought to laboratory, numbered, dried, de-haemoglobinised, fixed and stained in Giemsa stain.
The Duet(TM) had previously received FDA clearance for hematopoietic cells stained by Giemsa stain, Immunohistochemistry or ISH (with bright field and fluorescent) prepared from cell suspension for in-vitro diagnosis use, as an aiding tool to the pathologist in the detection, classification and counting of cells of interest based on particular color, intensity, size pattern and shape.
2 Reagent diluted Giemsa stain Manual 1000 360 pH = 6.
Each lymph node was serially sectioned and touch preparations were made and stained with H&E, modified Diff-Quik Giemsa stain, or both (based on an assigned pathologist preference) for intraoperative diagnosis.
As a loss of substrate adherence is an early event in apoptosis, we examined morphological alterations by Giemsa stain and internucleosomal DNA fragmentation by agarose gel electrophoresis.
On histologic examination, granules exhibited a typical, although faint, metachromasia with toluidine blue and Giemsa stains (Fig 2D).