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Yet another interesting modification to Gram's stain involves the addition of a fast green and tartrazine step before counterstaining with safranin.
Recently, it has been suggested that an organism's reaction in Gram's stain is not strictly a result of the chemical composition of its cell wall, but rather is directly related to cell wall thickness.
When all goes well, our beloved Gram's stain supplies crucial information leading to correct identification of isolates and provides rapid results to physicians waiting to treat seriously ill patients.
A reliable Gram's stain result begins with a properly made smear.
When a significant inflammatory response is present, organisms harvested for culture may already be so damaged that visualization via Gram's stain is impaired or even impossible.
No procedure in microbiology is more familiar to the lab professional than Gram's stain.
Fecal cultures are often used either in combination with fecal Gram's stain cytology or as a second tier diagnostic test to further evaluate potential pathogens identified on cytologic examination.
13) However, one disadvantage of using Gram's stains to evaluate apparently healthy birds is that Gram's stain results and clinical health or disease are not always in good agreement.
Sample material from each swab was then transferred directly to a microscope slide for a Gram's stain or used to inoculate sheep's blood and MacConkey agar plates for culture.
Attempts were made to differentiate each bacterial isolate to the species level, although, in some cases, the organisms could be categorized only to the generic level of Gram's stain morphologic features.
To investigate the level of agreement between results from Gram's stain and bacterial culture when used to assess cloacal or fecal samples from psittacine birds, samples were taken from 21 clinically healthy Hispaniolan Amazon parrots (Amazona ventralis) and tested by Gram's stain cytology and bacterial culture.