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The kappa coefficient value showing the strength of agreement between the results of direct microscopy using Gram stain and culture in this study was 0.
In the second study, performing additional PCR-based CSF testing for EV, HSV, and VZV, combined with arbovirus serology, increased the diagnostic yield by 20 percent in the culture/ Gram stain negative adult patients.
The first was the Gram stain, with which we were looking for epithelial clue cells.
Diagnosis was confirmed by Gram stain of bronchoalveolar lavage (BAL) fluid and sputum, which showed typical appearance of Nocardia species, and subsequent culture of Nocardia on blood agar plates, which showed chalky white wrinkly nocardial colonies.
Because of the established impact on outcomes associated with timely and accurate processing and reporting of preliminary blood culture Gram stain results, this study aimed to establish benchmarks for laboratories to apply and assess performance of this critical test.
The following case study reports the shortcomings of the Gram stain and culture methods in a bacterial meningitis case caused by Neisseria meningitidis that was subsequently identified using a PCR method.
Gram stain procedure that Gram-positive bacteria have a thicker peptidoglycan cell wall than Gram-negative bacteria.
However, with the recognition that BV is associated with a diverse group of organisms, many of which are difficult, cumbersome and costly to culture in the laboratory, it became increasingly more common to process these specimens by Gram stain alone, without culture (11).
A gram stain of wound exudate may reveal gram-positive 'box-car' shaped rods.
Characteristics that are important to note on the sputum Gram stain are very few squamous epithelial cells (less than 25 per low per field, or one to two per high oil field).
WASHINGTON -- Genital Gram stains alone lack the diagnostic ability to detect Chlamydia trachomatis and Neisseria gonorrhoeae infections, based on data from 1,511 emergency department visits, reported Dr.
A Gram stain involves taking a cervical specimen with a swab during a pelvic exam, placing it on a slide, and staining it with dye.