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constant |
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abiding changeless chronic consistency constans continual continually recurring continuing dependable durable entrenched eternal faithful firmus fixation habitual immortal immutable imperishable | In summary, this method is based on the experimental finding that the apparent Michaelis constant values depend on the concentration of the second substrate. The maximum velocity and apparent Michaelis constant for this reaction was approximately 328. This paper presents an undergraduate laboratory exercise that allows for the experimental determination of the typical enzyme-kinetics parameters [the Michaelis constant (KM), the maximum rate of the enzyme-catalyzed reaction (Vmax), the turnover number (kcat), and the catalytic efficiency of the enzyme system at low substrate concentrations (kcat/KM)] using a standard benchtop multi-cell UV-vis spectrophotometer. |
Michaelis constant |
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