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Using agarose gel electrophoresis, Northern blot analysis, and quantitative real-time PCR, we compared the stability of total RNA and specific mRNAs in blood collected in PAXgene and EDTA tubes.
6K human gene chip and further defined based on quantitative measurements of mRNA levels by Northern blot analysis.
Northern blot analysis studies were used to confirm that the mRNAs are concentrated in rat brain myelin [ILLUSTRATION FOR FIGURE 1 OMITTED].
Northern blot analysis using human LDHA cDNA as a probe showed that R51 cells did not express wild-type LDHA mRNA but did express a small amount of abnormal mRNA with a molecular mass slightly higher than that of wild-type LDHA (Fig.
We have yet to determine if the two other mRNAs identified by Northern blot analysis arise from distinct p53 homologue genes or are shortened versions of p120.
The specificity of the clones positive for the endostyle was confirmed by a Northern blot analysis.
Appropriate clones were selected, grown in LB medium with kanamycin, purified, and sequenced as described above to serve as DNA competitors to be used in C-PCR or as cDNA probes for Northern blot analysis as described below (Table 2).
Hybridization conditions and post-hybridization washes were identical to those used in the northern blot analysis.

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