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1990; Key and Akeson, 1990), a strategy we have employed here to obtain reagents that may be useful in tracking the development of the optic tectum and cerebellum in this species.
Following anesthesia, tissue containing the optic tectum and cerebellum was dissected from the crania of tadpoles and froglets and homogenized together in amphibian Ringer's solution.
By finding their optimal positions between these two gradients, retinal axons replicate in the optic tectum the same visual pattern that their cell bodies create in the retina itself, Bonhoeffer suggests.
1% fast green dye (Sigma) established that an injection of 4 [micro]L at an angle of approximately 45-50[degrees] relative to the top of the head and at a depth of 3-4 mm would partially fill the brain ventricle and expose cells in the forebrain and optic tectum to the injected solution.