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1990; Key and Akeson, 1990), a strategy we have employed here to obtain reagents that may be useful in tracking the development of the optic tectum and cerebellum in this species.
Bonhoeffer and his colleagues have used labeled antibodies to show that RGM levels increase steadily from the front to the back of an animal's optic tectum.
1% fast green dye (Sigma) established that an injection of 4 [micro]L at an angle of approximately 45-50[degrees] relative to the top of the head and at a depth of 3-4 mm would partially fill the brain ventricle and expose cells in the forebrain and optic tectum to the injected solution.