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| This method, based on eDNA representational difference analysis (cDNA RDA), uses 96 hexanucleotides that are not suitable for priming ribosomal RNAs but that normally prime most of the genome of an RNA virus as primers for reverse transcription in eDNA RDA (1). HCV is a small, enveloped RNA virus that is part of the Flaviviridae family. Named for its distinctive crown-like appearance on electron microscopy, it is a single-strand RNA virus that is found to cause up to 30 percent of cases of the common cold in the United States. |
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