The most characteristic cis-regulatory element, detected in the mGH, is the TATA box
(Goldberg-Hogness box), a binding site of the TATA-Binding Protein (TBP) that is part of the transcription preinitiation complex (Schluesche et al.
For construction of luciferase vector containing only promoter (-2,657 to +39) of porcine [beta]-casein gene, PCR was conducted using porcine [beta]-casein 5' arm DNA containing normal TATA or mutant TATA box
as a template.
The sequence also has a TATA box
located between 15 and 25 nucleotides in the 3' direction of the closest Dof element.
In our model, activation of the construct via promoter sites other than the EREs is avoided by using only a minimal TATA box
in the construct, resulting in low background activity.
41] However, Hoechst 33342-induced apoptosis is associated with mitochondrial dysfunction, inhibition of endogenous topoisomerase I activity,[25,26,40,41] and decreased formation of normal TATA box
-binding protein/ TATA box
complexes,[24,27] suggesting that Hoechst 33342-induced apoptosis is associated with a novel mechanism.