Experimental data from cultured human GCs from both control and PCOS women suggest that neither PI3K nor MAPK pathway are involved in insulin induced steriodogenesis (36) which is in contrast to theca cells
where PI3K has been shown to be involved (27).
The putative ligand-mediated activation of PPAR-[gamma]2 by troglitazone impairs androgen and stimulates progesterone biosynthesis in primary cultures of porcine theca cells
(113) by blocking the expression of the cytochrome P450-17-[alpha] hydroxylase/C17-20 lyase gene and CYP protein phosphorylation, which decreases the LH insulin-driven theca cell
One can argue that this spindle cell component is truly another pattern of granulosa cell tumor (pseudosarcomatous pattern) or it might represent a fibrothecomatous component, taking into consideration that most granulosa cell tumors have at least some theca cells
Augmented androgen production is a stable steroidogenic phenotype of propagated theca cells
from polycystic ovaries.
The creation of an artificial ovary marks the first time researchers have successfully created a three-dimensional environment that contains the three main types of ovarian cells: theca cells
, granulose cells, and the eggs or oocytes.
For this work, the researchers started with theca cells
, which form the outer coating of the follicle that holds the egg and produces a precursor to oestrogen.
Generally, it is more difficult to maintain a three-dimensional structure between the oocyte and granulosa cells in the enzymatically isolated preantral follicles because theca cells
and the basal lamina are partially damaged by collagenase.
The number of proliferating and non-proliferating cells was determined for granulosa and theca cells
of secondary (n=99 total) and antral (n=45 total) follicles.