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In a further investigation for the underlying molecular mechanisms, we determined whether MAPKs were activated in hispolon-treatedHONE-1 and NPC-039 cells by a Western blot analysis (Fig.
Downregulation of PCNA and calbindin-D9k protein levels after BPA exposure were confirmed by Western blot analysis (see Supplemental Material, Figure S1B,C).
Sc] in skeletal and heart muscle by Western blot analysis with sodium phosphotungstic acid precipitation for enrichment of [PrP.
We examined selectin levels through Western Blot analysis in HPV positive and HPV negative cell lines.
A visual comparison of the band intensities was performed by a western blot analysis (Fig.
For Western blot analysis, the same procedure was taken as described for anti- His antibody with minor differences.
Western blot analysis confirmed the presence of fragments in the 37 kDa and 35 kDa ranges, which were identified as angiostatin and mini-plasminogen by mass spectrometry.
Expression of MOD-5-VH fusion protein was verified by Western blot analysis using anti-V5 monoclonal antibodies where ~64 and ~83 kDa bands were detected representing the multiple glycosylated forms of MOD-5-VH.
The active cathelicidin protein was then detected in sebaceous glands using the anti-LL-37 immunoglobulin and by western blot analysis.
For Western blot analysis (n = 5), peptide fractions obtained from affinity chromatography were incubated overnight at 37 [degrees]C with 0.
Western blot analysis showed that the magnitude of the protein changes correlated with the severity of disease, with more expression seen in patients with high-volume disease.
Moreover, Western blot analysis showed that the magnitude of protein changes correlated with the severity of disease, with more expression seen in high-volume disease.