Embryonic
callus induction medium II: Orchimax minerals and vitamins, 400 mg/l casein, 2.5 mg/l 2,4-D, 1 g/l activated charcoal and 2 g/l gelrite.
With respect to the induction of adventitious shoots, it was observed that they were formed from the green spots originated from the
callus formation (Figure 1H).
Identification of Embryonic and Nonembryogenic
Callus. Difference between nonembryogenic and embryogenic
callus was achieved on the basis of
callus physical characteristic [21, 22].
An average of 6.4 shoots, 5.4 rooted seedlings and 3.8 plants were subjected to acclimatization and three adult acclimatized plants per gram of
callus were produced.
(2.) Kim SH, et al,
Callus formation is associated with hyperproliferation and incomplete differentiation of keratinocytes, and increased expression of adhesion molecules.
In order to determine the best
callus induction medium and type of explant, the experimental design was completely randomized, represented by 20 media types and two explants types.
Sterilized seeds were cultured on MS media (Murashige and Skoog, 1962) supplemented with 3% sucrose, 0.3% gelrite and 2 mg L-1 2, 4-D (pH 5.8) to induce the formation of MR219
callus. The cultures were maintained in the dark at 25 +- 2degC for 30 days.
usitatissimum from
callus culture under given in vitro conditions (medium supplemented by Ag NPs or Au NPs).
However, the application of tissue culture technology will depend on the availability of an effective regeneration system through
callus and/or shoot tip culture.
Although many methods of
callus removal are available, including autolytic therapy with hydrogels, whirlpools, and wet-to-dry dressings, Dr.