These are suitable conditions for sperm cell
capacitation 10 and also to evaluate their ability to undergo acrosome reaction.
Reactive oxygen species as mediators of sperm
capacitation and pathological damage.
(11) Studies have shown that [O.sup.-.sub.2] production is one the first events of
capacitation and the involvement of ROS generated in adenylyl cyclase and tyrosine activation is clearly demonstrated.
A shift in the balance of the oxidoreductive system is necessary to trigger the
capacitation.
There are four critical steps that are involved in a successful fertilization process: sperm maturation,
capacitation, hyperactivation, acrosome reaction, and sperm oocyte fusion [87, 98].
With the bulwark of their intellectual
capacitation, they exemplify the hope of our nation's redemption.
Gut rhetorics, accordingly, compose bodies and microbes as ruminations, coughing up the
capacitation of rhetoric, a kind of chewing, as well as chewing the cud, that is, thinking with/in the gut.
Combined Hoechst 33258/chlortetracycline fluorescence (H33258/CTC) staining is widely used method to directly measure the
capacitation status of spermatozoa by monitoring calcium-regulated changes using the fluorescent antibiotic chlortetracycline [12,13].
replaced with a new endocentric economic system that is based on African development
capacitation, resource-based industrialization and domestic prosperity generation, Africa cannot achieve the objectives of Agenda 2063."
On the other hand, excessive ROS production lead to oxidative stress, resulting in decreased sperm motility, viability, and increased midpiece sperm defects that impair sperm
capacitation and acrosome reaction [6].
The remaining pellet was resuspended by pipetting with 1 ml of medium and incubated at 37[degrees]C for 1 hour (
capacitation).