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acid |
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The osteoclastic
origin of the giant cells was confirmed by positive staining with
tartrate-resistant acid phosphatase (figure 2, B) in combination with
negative reactions for S100 and CD1a; these findings also excluded
Langerhans'-cell histiocytosis. In contrast to other
prostate-related antigens, such as prostate-specific antigen (PSA),
prostatic acid phosphatase (PAP) and prostate secretory protein, PSMA is
a membrane glycoprotein that is not secreted. Cultures were incubated at 37[degrees]C for 6-7
days and then fixed and stained for tartrate-resistant acid phosphatase
(TRAP) using the leukocyte acid phosphatase kit (Sigma). |
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