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Upon harvesting, 25 [micro]L of broth was added to 2 mL of brain-heart infusion broth, incubated for 4 h, and plated on cetrimide, Chapman, and bile-esculin-acid agar containing 10 mg/L of vancomycin (after an enrichment step of 18 h in broth containing 1 mg/L of vancomycin) to detect Pseudomonas aeruginosa, Staphylococcus aureus, and vancomycin-resistant enterococci, respectively. For feces, aliquots were plated on Chromagar, Cetrimide (Bio-Merieux), and Chapman agar for detection of yeasts, Pseudomonas aeruginosa, and S. The membranes were placed on Pseudomonas cetrimide fucidin cephalosporin (CFC) agar (Oxoid) and incubated at 30[degrees]C for 48 h. |
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