acid

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by bleaching, or dying via the oxidization of cystine linkage, forming mainly cysteic acid, resulting in a high number of anionic groups.
The peak separation is >95% on common amino acids profile (up to 45 amino acids in a single run with Lithium systems), including Taurine, Ornithine, oxidized amino acids such as Cysteic acid and Methionine Sulfone, and hydroxylated amino acids such as Hydroxyproline and Hydroxylysine.
Under these experimental conditions, the cysteinyl residue of the [alpha]T-12 peptide was oxidized to a cysteic acid residue, yielding a mass difference of -47.