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Cystine was determined as cysteic acid and methionine as methionine sulfone after preoxidation with performic acid and precolumn derivation using phenylisothiocyanate (L-8800 Hitachi Automatic Amino Acid Analyzer, Tokyo, Japan).
As disulfide bonds photodegrade, hair experiences a loss in cystine content and a corresponding increase in cysteic acid.
Methionine + cysteine content were determined as methionine sulphone + cysteic acid with separate samples hydrolyzed for 24 hours as described above after oxidation with performic acid overnight at 0[degrees]C (Moore, 1963).