The standardized cry4A product migrated to a position during DGGE equivalent to a denaturant
concentration of 53.
1981 forward: Calculated as fuel ethanol feedstock plus denaturant
minus fuel ethanol production.
Unlike commonly used paint denaturants
, which contain man-made polymers that are melamine-formaldehyde based or are made from petroleum-based raw materials, PPG's patented BC4200NP denaturant
uses a natural by-product of food processing as a primary ingredient.
Detection by denaturant
gradient gel electrophoresis of tumor-specific mutations in biopsies and relative bronchoalveolar lavage fluid from resectable non-small cell lung cancer.
See Biomass, Fuel Ethanol, and Fuel Ethanol Minus Denaturant
3] Nonstandard abbreviations: DGGE, denaturing gradient gel electrophoresis; CDGE, constant denaturant
gel electrophoresis; SSCP, single-strand conformational polymorphism; dHPLC, denaturing HPLC; and [T.
The use of constant denaturant
capillary electrophoresis (CDCE) (10) to detect mutations is well suited for the analysis of pooled samples (11).
DGGE, as described originally (1), relies on the partial denaturation of a DNA duplex, which slows its movement in an increasing gradient of denaturant
The UTMS will employ microfabrication technology, robotics, and Peoples Genetics' patented analytical technique, constant denaturant
capillary electrophoresis, in order to provide the throughput necessary to discover and quantify mutations throughout the entire length of genome.
Peoples Genetics is scaling its discovery operation around the HTMS instruments, which separate mutated DNA molecules through a high-resolution process, Constant Denaturant
Capillary Electrophoresis (CDCE).
Molecular screening strategies, including single-strand conformation polymorphism analysis (2), denaturing gradient-gel electrophoresis (3), constant denaturant
gel electrophoresis (4), or in vitro transcription/ translation assays (5), have been described and may facilitate the detection of mutations.
TTGE does not require the preparation of a chemical denaturant
gradient gel and can be performed without a GC clamp.