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denature
(redirected from denaturation)

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The product has no high temperature denaturation step or RNase solutions, and labware requirements, overnight hybridization and stringent wash conditions are eliminated.
Before we implement this new technology, we need to understand the mechanism and kinetics of pressure-induced degradation, denaturation, activation and inactivation of several food compounds, such as microorganisms, enzymes and nutrients, and the way in which degradation, denaturation and the like are influenced by other parameters, such as temperature and pH.
Parasite DNA was amplified after an initial denaturation at 95[degrees]C for 15 min; 43 cycles of denaturation at 95[degrees]C for 45 s and annealing at 60[degrees]C for 90 sec; and a final extension at 72[degrees]C for 5 min in an i-Q thermocycler (Bio-Rad, Hercules, CA, USA).
 
 
 
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