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The heating treatment was done in order to mimic one denaturing step of the PCR cycle in absence of primers.
This means that the typological method cannot throw light on the denaturing process.
Taking into account the time needed to set up and run the PCR and to perform DHPLC analysis, we were able to screen the entire coding region of the ATRX gene in ~16 h compared with 1 week for denaturing gradient gel electrophoresis analysis.
This system is ideal for laboratories currently using denaturing polyacrylamide gel system and silver staining or fluorescent dye labeling for genotyping with microsatellite markers.
Therefore, we combined with denaturing gradient gel electrophoresis (DGGE) to resolve this problem.
We have combined, in proof-of-principle experiments, the mismatch digestion properties of endo-VII with the high-throughput capabilities of MADGE and a newly developed denaturing MADGE format to create a simple mutation-scanning technique that can screen ~1000 PCR samples during a single 35-min electrophoretic run.
Incision reactions were performed at 37[degrees]C for 10 min, stopped by the addition of 10 [micro]L formamide buffer, and analyzed by electrophoresis on an 18% denaturing polyacrylamide gel.
Pediococcus, Leuconostoc and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis.
The European Commission was due on November 27 to give Italy the go-ahead to change the formula for a denaturing agent with a view to exempting anhydrous ethyl from excise duties.
When you cook an egg, you're denaturing the protein,' says Abergel.
Finally, because carnitine, leupeptin, taurine and valproic acid, individually, are all well known, safe and established molecules to the FDA, the Company believes they represent a low-risk development pathway as their combinations have shown no denaturing of the individual components.
To rationalise the Community system in relation to the denaturing of alcohol and in agreement with the European action plan to strengthen the fight against tax fraud and tax evasion, JRC will finalise the harmonisation of completely denatured alcohol and initiate actions on partly denatured alcohol on specific sectors exempted under 27(1)(b) which MSs deem to be priorities: a) screenwash, de-icer, thinner, anti-freeze and solvents; and b) cosmetics, perfumes and hygienic products