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Ultrathin (60 to 80 nm) sections were cut with a diamond knife on the ultramicrotome, collected on Formvar-coated single-slot grids, stained sequentially with 4% uranyl acetate and 1% lead citrate, and examined and photographed through an electron microscope.
The incision is made by a special diamond knife and there is no need of suture because the incision is so clear," Dr Akahoshi said.
After we selected areas to be examined, we cut ultrathin sections (50 nm) with a diamond knife.