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RC reference confirmation: a subsample of each Rapidcult[TM] enrichment culture was treated by immunomagnetic separation and streaking or spreading of the concentrate and supernatant onto Rainbow agar plates, as according to MLG 5.
After 6 consecutive transfers on Postgate's Medium B, all positive sulfate reducing enrichment cultures were examined for their ability to degrade TCE in the absence of sulfate.
For each enrichment, 20 [micro]L of Dynal anti-O157 paramagnetic beads should be combined with 1 mL of enrichment culture washed twice with PBS (pH 7.
Briefly, 4-5 loopful of stool sample was added to 10 ml of modified tryptic soy broth (TSB containing 20 mg/l novobiocin) for enrichment culture and incubated for 6-8 h at 37[degrees]C.
Considering only the results from initial blood samples, PCR detected Bartonella DNA in 3 samples, all of which were subsequently PCR positive by subculture or enrichment culture.
Anaerobic degradation of 2-methylnaphthalene by a sulfate-reducing enrichment culture.