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Construction of retroviral vector and determination of retroviral titre: After confirming orientation ofthe HSV-tk gene cloned in pLTKSN it was co-transfected into 293 cells with an ecotropic helper virus pEcoLandau to produce ecotropic viral particles.
In general, AAV2 requires association with a helper virus in order to replicate.
The helper virus carries the instructions for fabricating new virus outer coats, but cannot make copies of the virus' own genes.