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The free and bacterium-bound MAb fractions were eluted in ~15 min, with the MAb-bacterium immunocomplex eluted in a relatively broad band with a retention time of 8.
A positive result for scrapie is indicated by a dramatic reduction in the free peptide peak and a corresponding increase in the immunocomplex peak.
The bound immunocomplex was visualized with HRP-avidin-biotin complex using nickel-enhanced 3,3-diaminobenzidine as chromogenic substrate.
These results suggest the presence of an immunocomplex involving cTnI and IgG that produces the persistently high cTnI concentrations, probably because of the prolonged half-life of the IgG.