To conduct SRD, each laboratory must obtain all of the materials to create immunodiffusion
gels, thus analysis can be inconsistent between laboratories.
capsulatum, focusing on three distinct test groups: (i) antibody detection tests, including immunodiffusion
, complement fixation and latex agglutination; (ii) antigen detection tests, including enzyme immunoassays (EIAs); and (iii) molecular methods.
If the result is negative, it does not require confirmation, but if it is positive, it requires confirmation with immunodiffusion
in agar gel, which has a sensitivity of 71% (43).
Serodiagnosis of equine infectious anemia by agar gel immunodiffusion
and ELISA using a recombinant p26 viral protein expressed in Escherichia coli antigen.
However, the serum Coccidioides IgM and IgG by EIA were both positive, as well as Coccidioides immunodiffusion
for antibodies, but Coccidioides antibody complement fixation and pleural fluid Coccidioides antigen were negative.
Laboratory tests typically used for coccidioidomycosis diagnosis include complement fixation, immunodiffusion
, enzyme immunoassay, culture, histopathology, and PCR.
multocida have been identified using indirect hem-agglutination test (Carter, 1967; Rimler and Rhoades, 1987), while 1 to 16 somatic types have been identified by agar gel immunodiffusion
(AGID) test (Brogden and Packer, 1979).
Serologic tests for antibodies to Histoplasma and Blastomyces by immunodiffusion
Antibody testing: Aspergilus IgG, and Histoplasma, Blastomyces, Coccidioides, & Aspergillus by Immunodiffusion
plates were prepared using 1% agarose.
Serological diagnosis is usually performed using a tube agglutination test (TAT), a rapid slide agglutination test, and a gel immunodiffusion
IgA and IgG were determined by radial immunodiffusion