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Tape impression smears collected from ears revealed budding yeast cells under oil immersion objective after staining with new methylene blue (Fig.
Intestinal impression smears of the later (06 animals) were found as positive for the incidence of acid fast bacilli and later on, their tissue sections were cautiously examined in the laboratory.
Impression smears from the liver, spleen, lungs, and rectum were prepared and stained with a quick stain (Hemacolor, Merck, Darmstadt, Germany).
Most dermatology texts rely heavily on images, not only to identify the disease in the animal but also to illustrate diagnostic samples from tests, such as tape prep examinations and impression smears.
Brain impression smears from the four were negative for lyssavirus antigen by FAT.
Peripheral blood smear prepared from ailing animals and impression smears of liver, kidneys, spleen and lungs were taken for bacteriological examination.
Impression smears from cut sections of lungs, liver and heart blood smears were methanol fixed and stained with Giemsa and Grams stain.
Scabs were soaked overnight in sterile water for sufficient moistening so that under surface of scab was used to make effective impression smears by firmly pressing this surface on to microscope slide.