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A confirmed case was defined as illness in a patient who had a seroconversion or [greater than or equal to] 4-fold change in antibody titer between acute-and convalescent-phase serum specimens (by indirect fluorescent antibody or enzyme-linked immunosorbent assay), a positive polymerase chain reaction assay with primer pairs directed to genomic sequences specific to HGE, or detection of the specific 44-kDa protein band by Western blot analysis.
In our laboratory, we use the indirect fluorescent antibody test (IFAT) for antigens of the four plasmodia species, in addition to PCR screening.
On day 12, no cytopathic effect was observed, but a dengue 1 virus was identified by indirect fluorescent antibody assay (2) with type-specific monoclonal antibodies.