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Results with indirect fluorescent antibody stains of the agent cultivated in yolk sacs.
Results with indirect fluorescent antibody stains of the agent cultivated in yolk sacs: Summary of results with patients with Philadelphia respiratory disease
5 Tricuspid - 1289 10 Tricuspid - 116 6 Pulmonary - 379 8 Aortic - Indirect fluorescent antibody titer B.
Serum specimens assayed in parallel, in serial fourfold dilutions, by indirect fluorescent antibody testing for antibody to B.
The laboratory tests CDC offers for babesiosis, when indicated, include examination of blood smears, hamster inoculation, and PCR (5) for parasitologic diagnosis and an indirect fluorescent antibody assay for total immunoglobulin for serologic diagnosis (4).
The indirect fluorescent antibody (IFA) and the indirect hemagglutination (IHA) tests are the most sensitive and specific of these tests (4,5).
A confirmed case was defined as illness in a patient who had a seroconversion or [greater than or equal to] 4-fold change in antibody titer between acute-and convalescent-phase serum specimens (by indirect fluorescent antibody or enzyme-linked immunosorbent assay), a positive polymerase chain reaction assay with primer pairs directed to genomic sequences specific to HGE, or detection of the specific 44-kDa protein band by Western blot analysis.
A case is considered serologically confirmed if testing reveals an indirect fluorescent antibody (IFA) titer of [is greater than or equal to] 1:64, a complement-fixation (CF) titer of [is greater than or equal to] 1:16, or a fourfold rise in titer by the CF, IFA, microgglunatination (MA), latex agglutination (LA), or indirect hemagglutination (IHA) assays.
In our laboratory, we use the indirect fluorescent antibody test (IFAT) for antigens of the four plasmodia species, in addition to PCR screening.
On day 12, no cytopathic effect was observed, but a dengue 1 virus was identified by indirect fluorescent antibody assay (2) with type-specific monoclonal antibodies.