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Unlike several other NTD-inducing teratogens (Mirkes 2002), we found that VPA induced no apparent increase in apoptosis along the tips of the neural folds (Copp et al. In neural plate stage embryos (stages 15-18) hybridized with antisense probes, staining was observed along the neural fold regions corresponding to premigratory cranial neural crest for control, vehicle alone (Figure 8A), nonylphenol (Figure 8C), and methoxychlor (data not shown) treatments. |
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