culture

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The plate culture of all isolates prepared in Starch casein medium was observed for morphological characteristics like colony characteristics, coloration, margin etc.
Mycelia were removed from plate culture, ground in a sterile tissue homogenizer, and transferred to a 1-L Erlenmeyer flask containing 500 mL of Schenk and Hildebrandt basal salts and vitamin (Sigma Chemical Company, St.
If a plate culture is prepared, the plate should be sealed with adhesive tape once growth is evident, and the culture plate should be destroyed after 10-14 days.