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Porcine stress syndrome (PSS) susceptibility, has been associated with a single point mutation C1843T (Arg615Cys) in the Ca2+ release channel at the sarcoplasmic reticulum ryanodine receptor 1 (RYR1).
Current methods for genotyping the C1843T Porcine stress syndrome (PSS) susceptibility mutations include allele-specific oligonucleotide hybridization and PCR restriction fragment length analysis, and these methods require multiple manual steps and are time consuming (5,15).
Finally, we have demonstrated a 100% concordance between the in vivo and in vitro porcine stress syndrome (PSS) susceptibility results and the ones obtained by means of molecular genotyping such as real-time PCR using fluorescein-labeled hybridization probe, sequencing and restriction analysis.

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