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postfix |
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The remaining tissue was postfixed in 4% paraformaldehyde for immunohistochemistry and stereologic counts. For thin-section electron microscopy, infected cells were fixed in cacodylate-buffered glutaraldehyde (2%), scraped from the flask, postfixed through increasing concentrations of ethanol (to 100% vol/vol), and then araldite embedded. The segments were postfixed in 1% osmium tetroxide in 0. |
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