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Various marker systems for evaluating microbial nitrogen (MN) in the rumen were developed, such as purine bases (PB) and nitrogen isotope ([sup.
Low sensitivity in predicting total PD excretion from spot urine collected between 08:00 to 09:30 h may indicate that this time span fell, or partly fell in the lag phase for the hydration and colonization of feed particles by rumen microbes during which time the supply of MN as well as microbial purine bases cannot be properly reflected by the concentration of PD in spot urine.
As described earlier, creatinine excretion is usually independent of the level of intake or duodenal infusion of purine bases (IAEA, 2002).