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site noun address, base, environs, habitat, locale, location, neighborhood, place, position, setting, situs, stead, territory, vicinity site verb arrange, assign, base, center, localize, locate, place, position, situate See also: building, circuit, habitation, locality, location, part, pinpoint, place, scene, seat, situs, structure, venue How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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Plasmids containing cDNA from human 17[beta]-HSD1 or 17[beta]-HSD2, kindly provided by Stefan Andersson, were recloned into pcDNA3 vector by PCR with primers at the 5' end containing a HindIII restriction site (17[beta]-HSD1) or a BamHI restriction site (17[beta]-HSD2), a Kozak consensus sequence (Kozak 1989) and the initiation codon, and primers at the 3' end containing a stop codon followed by an XbaI restriction site. The observed change in restriction pattern is the result of a mutation of the BamHI restriction site at [approximately equal to] 0. The G to A mutation in SULT1A1 removed the restriction site for the endonuclease HaeII. |
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