Assembly was achieved in the pFastBac1 donor plasmid by exploiting unique restriction sites
to concatenate the entire genome from 3 sub-clones.
The insertion of the KpnI restriction site
allowed the circularization of the linear amplicon.
The nature of the TRFLP analysis makes definitive identification of TRFs difficult, because numerous unrelated bacteria groups may share restriction sites
, and fluorochrome dye labels and purine content can influence sizing errors during analysis (Schutte et al.
This enzyme has a unique restriction site
at base 170 in the Fayoumi line.
6] paired-end reads, 97% of which were mapped to restriction sites
with the predicted fragment length and used for subsequent analysis (see Table 1 in the online Data Supplement).
The presence of a restriction site
is denoted by a "1", while "0" denotes the absence of the site.
Methods for DNA extraction and restriction site
analysis are the same as in earlier studies (Soreng et al.
Thus, when a mutation that creates a restriction site
is present, the fragment will be cut; if the mutation destroys a restriction site
, it will not be cut.
Tell students that the Shh gene has a SaclI restriction site
at base pair #19 (SacII is another restriction enzyme).
Authentication of canned tuna and bonito by sequence and restriction site
analysis of polymerase chain reaction products of mitochondrial DNA.
The sequences from the four species of BF cirrhosae have the restriction site
(CCC[and]GGG) of endonucleases SmaI, while other species from the Fritillaria genus do not have this sequence in the same site (Fig.
Hinf I Species Restriction site
Fragments Pattern Paranthrene simulans -- (a) 606 A Vitacea polistiformis 203 203, 403 B Melittia satyriniformis 102 102, 504 C Pennisetia marginata 102 102, 504 C Synanthedon rileyana 15, 102, 270 87, 168, 336 D S.