17) Briefly, for each sputum specimen
panels of four screw capped round bottom borosilicate glass tubes (Himedia, Mumbai) were used.
Similarly to previously reported data, [14,15] use of Xpert MTB/RIF on a single sputum specimen
in our study provided superior detection over smear microscopy.
Detection and identification of mycobacterium tuberculosis directly from sputum specimen
by Amplicore PCR.
Because of the difficulty in transporting sputum specimens
in many rural areas of the world, one novel approach is to ship sputum smears on slides to a central laboratory for testing.
The TB control plan also encourages that there be no delay in collecting sputum specimens
to rule out TB infection.
Of the three patients who had a mass visible on their chest radiograph and negative fibreoptic bronchoscopy and induced sputum specimens
, two had peripheral shadows which were later diagnosed by needle biopsy (1 adenocarcinoma and 1 small cell carcinoma), and on subsequent computerized tomography in the third there was no evidence of a tumour.
Ninety per cent of all sputum specimens
sent from all facilities in the Umzinyathi District (which Greytown M3 covers as an MDR-TB referral facility) were returned within 48 hours last year, allowing treatment of smear-positive patients to begin immediately.
All the sputum specimens
were examined for AFB by Z-N staining in the laboratory as per RNTCP guidelines.
18] The sensitivity of the test in that study, which included 569 sputum specimens
from 187 inmates with suspected TB in prison infirmaries or clinics, was higher than in the clinical trials (92.
were refrigerated at 4-6oC in cases where processing was delayed.
Each sputum specimen
was first examined microscopically in a direct smear prepared from the untreated specimen and then in a smear made from a centrifuged concentrated deposit obtained after the specimen was treated and homogenized (Fig.
Specimens Submitted for Acid-Fast Stain and Culture Number of Specimen Tested Patients Tested 3 Sputum specimens
3 2 Sputum specimens
2 1 Sputum specimen
7 Blood culture 13 Fine-needle aspirate of peripheral lymph node 5 Bone marrow aspirate or biopsy 5 Cerebrospinal fluid 4([double dagger]) Number of Number of Patients Patients With With Positive Positive Specimen Tested Acid-Fast Smears Cultures 3 Sputum specimens
1 1 2 Sputum specimens
1 1 1 Sputum specimen
3 4 Blood culture NA(*) 8 Fine-needle aspirate of peripheral lymph node 5 5 Bone marrow aspirate or biopsy 3([dagger]) 3([dagger]) Cerebrospinal fluid 0 4