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stopper |
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The swollen pieces were quickly removed from n-heptane, excess n-heptane blotted off and weighed in a stoppered preweighed vial. 5 mL) was pipetted into a graduated stoppered centrifuge tube, 6 mL of hexane was added, and it was rotated in a slow-speed Roto-rac (National Institute of Occupational Health, Ahmedabad, India) for 2 hr. Most blood-fed specimens were placed in stoppered vials and frozen at -70 [degrees] C until they were triturated and tested for virus. |
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