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Related to taurocholic acid: glycocholic acid
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In the 3 ml reaction mixture, potassium phosphate 287mM, taurocholic acid 0.
The enzymatic analysis of the individual biliary bile acids after separation by thin layer chromatography showed taurocholic acid as the major bile acid in all the bile samples (data not shown) although further studies are needed due by this technique it was not possible to detect other rat bile acids as [alpha]- and [beta]-muri-cholic acids (Scheibner et al.
A CBA (conjugated bile acids) kit was obtained from Sigma, and sodium salts of glycochenodeoxycholic acid, glycocholic acid, taurochenodeoxycholic acid, and taurocholic acid were used for this study.