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dose
(redirected from tissue culture infective dose)

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Dilutions of test serum (25 [micro]L) were incubated with one hundred 50% tissue culture infective doses of the virus in the same volume (25 [micro]L) for 1 h at 37[degrees]C in modified Eagle medium (5), after which 50 [micro]L of a suspension (2 x 104 cells/mL) of Vero cells plus fetal calf serum was added to the same medium to reach a final concentration of 5%.
The second virus passage was used for the experiments; 50% tissue culture infective dose (TCI[D.
 
 
 
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