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In a 96-well plate, 2-fold serially diluted serum samples beginning at 1:10 were incubated with 200 median (50%) tissue culture infective doses of pseudovirus at the final volume of 100 [micro]L at 37[degrees]C for 1 hour; then the mixture was added to the culture of MDCK cells.
Presentation Number V-1556: "Comparison of Tissue Culture Infective Dose (TCID50) and Quantitative PCR (QPCR) in the Diagnosis and Follow Up of Influenza Hospitalized Subjects"
For example, no decrease was observed in a 50% tissue culture infective dose (TCI[D.