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crystallized |
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After primary isolation, these viruses were further propagated in LLC-MK2 cells in opti-MEM (Invitrogen) with 2 [micro]g/mL trypsin (replenished every other day), 100 IU/mL of penicillin G sodium, 100 [micro]g/mL streptomycin, and no serum. III] tended to aggregate, were smaller in size than untreated cells (Figure 2), and required longer trypsin digestion to detach them from the surface (data not shown), indicating increased adhesion activity. The new investment provides ProdiGene with the resources to complete commercialization of trypsin and other products. |
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