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All serum samples underwent ELISA testing; positive results were confirmed by virus neutralization (4).
The virus neutralization assay was performed as described (4).
Serum samples from the 3-month-old puppy and the dam were tested by virus neutralization test (VNT), according to the protocol used for ruminant serum.
The diagnosis was confirmed with serologic test results, which were strongly positive in serum and CSF, and virus neutralization testing performed as described in Stiasny et al.
Virus-neutralization titers against WNV (strain Eg-101), BAGV (strain Spain/2010), and USUV (strain SAAR1776) were determined by micro virus neutralization test (VNT) as described (6).