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Ten colonies were randomly picked and sequenced; 9 clones were mutant, and 1 was wild type. Cells expressing each mutant accumulated MAs(III), although at a somewhat lower rate than wild type (Figure 5B). Now experimental and theoretical studies with wild type enzyme and an engineered mutant have shown that hydroxylation is regiospecific at the 1-position for a family of benzocycloarene compounds: benzocyclobutene, benzocyclopentene (indian), benzocyclohexene (tetralin), and benzocycloheptene. |
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