in both sheep and goats by real-time PCR assay.
suspensions were included in each series of tests.
in livestock has been eliminated, except in limited areas where disease reintroduction from infected wildlife occurs.
RBT was carried out according to 17 with Brucella abortus
S99 antigen (Spinreact SA, Gerona, Spain).
In this review, Brucella abortus
seemed to be the specie most associated with abortions in Africa (12,99,102,109).
Six days later, Brucella abortus
was isolated from both samples in the sheep's blood agar [35[degrees]C, 5-10% C[O.sub.2]] (figure 4).
Molecular Detection of Brucella abortus
DNA Extraction: DNA extraction was carried out from clinical samples and colony using DNeasy Blood and Tissue Kit (Qiagen) following manufacturers protocols.
Hastalarin % 3,9 'unda uc ya da daha fazla abortus
is the most important cause of bovine brucellosis, while both Brucella melitensis and Brucella ovis cause brucellosis in sheep.
poseen la capacidad de infectar aves y mamiferos (Frutos et al.
In humans Brucella abortus
causes undulant fever, an occupational disease of people working with animals such as veterinarians, farmers and abattoir personnel.