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The respiratory organs of air-breathing fish are the accessory organs of gill respiration.
They were then allowed for 255 hours (11.45 days) (being the wash-out time of amitriptyline) at the end of which they were again randomly selected and assessed for mating behavior, relative weight of sex and accessory organs, and hormonal profile.
The male reproductive system is comprised of two main elements; essential organs (gonads) for production of gametes and supporting accessory organs (penis), for transporting gametes to their destination (Anthony & Thibodeau 1979).
The species of clarias are widely distributed, being found in all freshwater areas, even in almost dry pools, as they have accessory organs to breath atmospheric air (Smith, 1945).