Treatment may have suppressed the organism substantially so that numbers were too low to be identified on acid-fast stain
or isolated on culture.
The positive acid-fast stain
from one of the calves and one of the students with a confirmed case implicated the obstetrics laboratory as the source of the outbreak.
Gram stain revealed no organisms; bacterial and mycobacterial cultures were negative and acid-fast stain
Demonstration of Charcot-Leyden crystals by acid-fast stains
applied on tissues.
The acid-fast stain
and culture examinations should be considered together to assess active TB in HIV patients.
In our case, the acid-fast stain
for mycobacteria was negative.
Gram stain (Figure, C) and a modified acid-fast stain
(Figure, D) revealed gram-positive, partially acid-fast filamentous organisms branching at right angles within the eosinophilic material consistent with Nocardia organisms.
The bug was considered a rare and exotic pathogen until the mid-1980s when a modified acid-fast stain
was employed to look for Cryptosporidium in stool specimens, especially from people with impaired immune systems who suffered from HIV disease.
Data of surgical pathology were obtained in seven patients, five cases presented with epithelioid granuloma with caseous necrosis with or without positive acid-fast stain
in bowel tissue; two cases presented with epithelioid granuloma with caseous necrosis with positive acid-fast stain
in mesenteric lymph nodes.
All state laboratories began routine testing with a modified acid-fast stain
As in many developing countries, the acid-fast stain
is the only bacteriologic basis for diagnosing tuberculosis (TB) in primary health care institutions in China, where facilities are limited for M.
In paraffin sections, the organism can be visualized by silver stains, tissue Gram stains, or Fite-Ferraco acid-fast stain