hypopitys commonly develops underground adventitious buds on the roots, which presumably contain an embryonic inflorescence , and, thus, the MhyYAB5 gene can be expressed in the buds.
hypopitys roots with adventitious buds; ab--adventitious bud, rs--growing reproductive stem; scale bar = 1 cm (c); relative expression (qRT-PCR) of the pattern of the MhyCRC, MhyINO1, MhyINO2, MhyFIL3, and MhyYAB5 genes in pinesap tissues, F--flower, B--bract, R--root (d).
In vitro induction and culture of adventitious buds
in epicotyl segments of sour orange.
Organogenesis is characterized by the formation of adventitious buds
. It is considered direct when bud regeneration occurs without callus, and defined as indirect when the regeneration process is preceded by callus formation, which develops into adventitious buds
that grow into new organs (Phillips, 2004).
Later, a few adventitious buds
developed from the callus at the edge of cut surface (Table 1).
Additives promote adventitious buds
induction from anther culture of Bitter melon (Momordica charantia L.).
Many adventitious buds
are formed on the margins or the inner regions of cotyledon explants after 3 weeks incubation on medium containing BA (1.5 mg [l.sup.-1]) and IBA (0.05 mg [l.sup.-1]).
According to Grattapaglia and Machado (1998) BA (6-benzilaminopurine) is the cytokine that provides best results on in vitro aerial parts multiplication and adventitious buds
For elongation of shoots, the calli with adventitious buds
divided into several sections (each section containing 3-5 adventitious buds
) were transferred to MS medium supplemented with [GA.sub.3] (1.0-3.0 mg/l).
Sugars, Hormones, Environment Affect the Dormancy Status in Underground Adventitious Buds
of Leaf Spurge (Euphorbia esula).