Since the EURL ECVAM database only includes substances that are positive in the
Ames test, the ECHA database (1) was used as a second information source.
Caption: FIGURE 12: Results from the decision tree for estimating in vitro mutagenicity (
Ames test): bisoprolol API (a) and bisoprolol Imp.
The scholars that highlight
Ames test limitations point to the fact that it is utilizing bacteria that is very different from human cell and therefore advocate for models using human or mammalian cells.
In
Ames test the mutant strain of Salmonella typhimurium was used.
For the P pentosaceus strain, there were few researches who had evaluated its safety with the
Ames test and a 28-day feeding test.
typhimurium
Ames test. These results suggest that the LME and SME of Anemopsis californica may be safe for use in humans and should be considered for further medical development studies.
a) The concentrated water extracts after the chlorination process were mutagenic to bacteria and genotoxic for mammal cells (human lymphocytes), evaluated through the
Ames test and comet assay, respectively.
The mutagenic activity was determined by applying the Salmonella/microsome test, known as the
Ames test, using TA98 and TA100 strains of S.
Because of this, in the present study we used two test systems: the prophage [lambda] induction (SOS-Inductest) and the bacterial reversion assay (
Ames test).
This was accomplished by searching each database for "
Ames test OR Salmonella mutagen." This gave 11,064 responses in PubMed, 13,694 in Scopus, and 3,453 in the Web of Knowledge.
The first ISO option is to perform the
Ames test, mouse lymphoma and a third chromo some aberration test that eliminates the need for colony sizing.
Where the negative
Ames test result has proved to be a false indication of safety, the compound must be discarded.
