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Precast commercial polyacrylamide gels for separation of DNA amplificates by temperature gradient gel electrophoresis: application to clonality analysis of lymphomas.
In addition to the routine genotyping at codons 136154 and 171, PRNP gene sequences from the 2 scrapie cases were determined by sequencing of PCR amplificates from brain-derived DNA to compensate for the possibility of a genetic chimerism in blood.