ICE

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ICE

abbreviation for INSTITUTE OF CHARTERED ENGINEERS.
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Real-time polymerase chain reaction (PCR) was employed to evaluate the relative mRNA expression level using the following primers: BAG1 forward: 5'-CAGGGCAGCAGTGAACCA-3', and reverse: 5'-ACCCGGCAACCATCTTGT-3'; BCL2 forward: 5'- CTGGGAGAACAGGGTACGATAA-3', and reverse: 5'-GGCTGGGAGGAGAAGATGC-3'; TP73 forward: 5'-CTCGGGAGGGACTTCAACG-3', and reverse: 5'-CCTGTGGTGGCTCATAGG-3'; CASP1 forward: 5'-ATTACAGACAAGGGTGCT-3', and reverse, 5'-GAATAACGGAGTCAATCAAA-3'; LTBR forward: 5'- CAAAGATGAAGTTGGGAAGG-3', and reverse: 5'- GTTTCCTGCAGAGAGAAGGGTGGCT-3'; CASP4 forward: 5'- GCACAATGGGCTCTATCT-3', and reverse: 5'-CAGTCGTTCTATGGTGGG-3'; GAPDH forward: 5'-AAGAGCACAAGAGGAAGAGAGAGAC-3', and reverse: 5'-GTCTACATGGCAACTGTGAGGAG-3'.
Caption: FIGURE 1: The change of the coefficient of variation CV for 22 proteins: APOL6, CASP1, CNP, CXCL10, DHX58, DRAP1, DYNLT1, FAM46A, GEMIN4, GORASP1, IFITM1, IRF7, PLSCR1, RTP4, SAMD9, SAMHD1, SIGLEC1, TAF1C, TLR7, TNFAIP6, TREX1, and ZBP1, The %-axis denotes the time (unit: h), It indicates that as the critical transition occurs, that is, the time evolves towards t = 45 h (see the vertical black line), the coefficient of variation CV for 22 proteins above significantly increases in the symptomatic group and has no obvious change in the asymptomatic group, Therefore, these 22 proteins can be viewed as DNBs for early-warning signals,
Similar changes were observed in the hepatic gene expression of Pycard, Casp1, and Il1[beta], which were increased in WT, but no or significantly less in Pr[2x.sub.7.sup.-/-] on a HFD (Figures 6(a), 6(b), and 6(c)).